问答题

(南京大学2008年考研试题)E.Coli cells are growing in a medium containing lactose but no glucose,indicate whether each of the following changes or conditions would increase,decrease,or not change the expression of the lac opern.It may be helpful to draw a model depicting what is happening in each situation. (a)addition of a high concentration of glucose (b)a mutation that prevents dissociation ofthe Lac repressor form the operator (c)a mutation that completely inactivates β-galactosidase (d)a mutation that completely inactivates galactosied permease (e)a mutation that prevents binding of CRP to its binding site near the lac promoter

【参考答案】

正确答案: (a)随着葡萄糖浓度增加,cAMP含量减少,低浓度的cAMP使C反应蛋白不能绑定来帮助RNA聚合酶结合到启动......

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