问答题
试说明RFLP的基本原理,及其在现代遗传和人类疾病诊断过程中的应用。
【参考答案】
RFLP(Restriction Fragment Length Polymorphisma,限制性片段长度多态性)。R......
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Using interrupted mating experiment, 5 Hfr strains (1, 2, 3, 4 and 5)were inspected to infer the sequence that these strains transfer several different genes (F, G, O, P, Q, R, S, W, X and Y)to an F-strain. According to the results, any strain had its particular transferring sequence, as follows (only the first 6 genes transferred are cited for any strain): (1)What are the gene sequences of the original strains of these Hfr bacteria (2)In order to obtain a highest rate Hfr recombinant, which marker gene of the donor should be selected in the recipient after conjugation
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问答题
An ordinary scientist conducted a cross of shmoos double heterozygote with the same genotype AaBb. The phenotypic segregation rate of the filial generation he detected is 9:7. He was sure this is not a phenomenon of double recessive epistasis and it should be involved in a segregation rate of 9:6:1, and however, aabb is lethal. He thinks it would not be realizable to distinguish 9:7 and 9:6 by statistic method. but he cannot solve this problem. Can you help him
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